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Ultra-long-working-distance multiphoton objective unlocks new possibilities for imaging

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In 1858, the first standard for microscope objectives was established to encourage interchangeable components. Over the following 150 years, standards have evolved to constrain the size of objectives, which limits the parameters of working distance, field of view and resolution. A new design breaks out of this conventional envelope, offering an ultra-long working distance in air and enabling new neuroscience experiments.

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Fig. 1: The Cousa objective.

References

  1. Yu, C. H., Stirman, J. N., Yu, Y., Hira, R. & Smith, S. L. Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry. Nat. Commun. 12, 6639 (2021). This paper reports a custom-designed large-field-of-view multiphoton microscope unconstrained from the conventional parameters.

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  2. Voigt, F. F. et al. Reflective multi-immersion microscope objectives inspired by the Schmidt telescope. Nat. Biotechnol. https://doi.org/10.1038/s41587-023-01717-8 (2023). This paper reports a design of multi-immersion multiphoton objective in an unconventional form factor.

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  3. Millett-Sikking, A. & York, A. Preprint at Zenodo, https://doi.org/10.5281/zenodo.3376243 (2019). This preprint paper reports an objective design with a protruding, angled tip optimized for oblique-plane light-sheet imaging.

  4. Sapoznik, E. et al. A versatile oblique plane microscope for large-scale and high-resolution imaging of subcellular dynamics. eLife 9, e57681 (2020). This paper reports a microscope system equipped with the objective design described in ref.3.

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This is a summary of: Yu, C.-H. et al. The Cousa objective: a long-working distance air objective for multiphoton imaging in vivo. Nat. Methods https://doi.org/10.1038/s41592-023-02098-1 (2023).

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Ultra-long-working-distance multiphoton objective unlocks new possibilities for imaging. Nat Methods 21, 24–25 (2024). https://doi.org/10.1038/s41592-023-02116-2

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