Fluorescent actinometers enable the measurement of light intensity even in the depths of samples and over wide ranges of wavelengths and intensities. We introduce two protocols to quantitatively characterize the spatial distribution of light of various fluorescence imaging systems and to calibrate the illumination of commercially available instruments and light sources.
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References
Boehm, U. et al. QUAREP-LiMi: a community endeavor to advance quality assessment and reproducibility in light microscopy. Nat. Methods. 18, 1423–1426 (2021). An article that introduces the QUAREP-LiMi community, which is concerned with the measurement of the absolute flux of light at the sample and the assessment of the uniformity of illumination.
Kuhn, H. J., Braslavsky, S. E. & Schmidt, R. Chemical actinometry (IUPAC technical report). Pure Appl. Chem. 76, 2105–2146 (2004). A review that introduces actinometry and its applications.
Valeur, B. & Berberan-Santos, M.-N. Molecular Fluorescence: Principles and Applications 2nd edn (Wiley, 2012). A book that covers the multiple aspects of fluorescence spectroscopy.
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This is a summary of: Lahlou, A. et al. Fluorescence to measure light intensity. Nat. Methods https://doi.org/10.1038/s41592-023-02063-y (2023).
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Fluorescent actinometers for fast and simple quantitative measurement of light intensity. Nat Methods 20, 1881–1882 (2023). https://doi.org/10.1038/s41592-023-02062-z
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DOI: https://doi.org/10.1038/s41592-023-02062-z
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