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A practitioner’s view of spectral flow cytometry

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Spectral flow cytometry enables the simultaneous analysis of a large number of cell surface markers at the single-cell level

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Fig. 1: Theoretical distribution of spectra for 21 fluorophores used in a human CD4 T cell profiling experiment on a five-laser SF analyzer.
Fig. 2: Gating and unmixing are linked steps of SF data analysis.

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Acknowledgements

We thank the entire staff of the Scripps Research flow cytometry facility. This work was supported by grant NIDDK 1R01DK117138 to L.T. and a Clinical and Translational Science Award issued to the Scripps Translational Science Institute TL1TR002551 to S.S.

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Authors and Affiliations

  1. Department of Immunology and Microbiology, Scripps Research, La Jolla, CA, USA

    Siddhartha Sharma, Josh Boyer & Luc Teyton

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  1. Siddhartha Sharma
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  2. Josh Boyer
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  3. Luc Teyton
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Correspondence to Luc Teyton.

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Nature Methods thanks David Parks and the other, anonymous, reviewer(s) for their contribution to the peer review of this work.

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Sharma, S., Boyer, J. & Teyton, L. A practitioner’s view of spectral flow cytometry. Nat Methods 21, 740–743 (2024). https://doi.org/10.1038/s41592-023-02042-3

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