We introduce GelMap, a flexible workflow for reporting deformations and anisotropy in expansion microscopy. By intrinsically calibrating the expansion hydrogel using a fluorescent grid that scales with expansion and deforms with anisotropy, GelMap enables the reliable quantification of expansion factors and correction of deformations.
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References
Chen, F., Tillberg, P. W. & Boyden, E. S. Expansion microscopy. Science 347, 543–548 (2015). This landmark paper introduces ExM and provides the foundation for subsequent work.
Truckenbrodt, S. Expansion microscopy: superresolution imaging with hydrogels. Anal. Chem. 95, 3–32 (2023). This review presents an extensive overview of the chemistry of ExM, highlighting recent advances and challenges.
Zhao, Y. et al. Nanoscale imaging of clinical specimens using pathology-optimized expansion microscopy. Nat. Biotechnol. 35, 757–764 (2017). This paper presents a clinically optimized variant of ExM and highlights the potential of ExM for use in diagnostic applications.
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This is a summary of: Damstra, H. G. J. et al. GelMap: intrinsic calibration and deformation mapping for expansion microscopy. Nat. Methods https://doi.org/10.1038/s41592-023-02001-y (2023).
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Mapping deformations and increasing quantitative accuracy in expansion microscopy. Nat Methods 20, 1460–1461 (2023). https://doi.org/10.1038/s41592-023-02015-6
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DOI: https://doi.org/10.1038/s41592-023-02015-6
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