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Discovering CRISPR–Cas off-target breaks

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DISCOVER-seq+ enables the efficient detection of CRISPR–Cas off-target breaks in primary cells and tissues.

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Fig. 1: DISCOVER-seq+ increases the detection of CRISPR–Cas off-target sites in a variety of model systems.

References

  1. Katti, A., Diaz, B. J., Caragine, C. M., Sanjana, N. E. & Dow, L. E. Nat. Rev. Cancer 22, 259–279 (2022).

    Article  CAS  PubMed  Google Scholar 

  2. Zou, R. S. et al. Nat. Methods https://doi.org/10.1038/s41592-023-01840-z (2023).

    Article  PubMed  PubMed Central  Google Scholar 

  3. Tao, J., Bauer, D. E. & Chiarle, R. Nat. Commun. 14, 212 (2023).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Kim, D. et al. Nat. Methods 12, 237–243 (2015).

    Article  CAS  PubMed  Google Scholar 

  5. Tsai, S. Q. et al. Nat. Methods 14, 607–614 (2017).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Wang, X. et al. Nat. Biotechnol. 33, 175–178 (2015).

    Article  CAS  PubMed  Google Scholar 

  7. Tsai, S. Q. et al. Nat. Biotechnol. 33, 187–197 (2015).

    Article  CAS  PubMed  Google Scholar 

  8. Frock, R. L. et al. Nat. Biotechnol. 33, 179–186 (2015).

    Article  CAS  PubMed  Google Scholar 

  9. Akcakaya, P. et al. Nature 561, 416–419 (2018).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Crosetto, N. et al. Nat. Methods 10, 361–365 (2013).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Yan, W. X. et al. Nat. Commun. 8, 15058 (2017).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Wienert, B. et al. Science 364, 286–289 (2019).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. Kaya-Okur, H. S. et al. Nat. Commun. 10, 1930 (2019).

    Article  PubMed  PubMed Central  Google Scholar 

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Acknowledgements

We thank M. Bienko (Human Technopole and Karolinska Institutet) for providing insightful comments and suggestions. This work was supported by funding from the Swedish Research Council (grant. no. 2022-00721) to N.C. and by intramural funding from Human Technopole to C.J. and N.C.

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Authors and Affiliations

  1. Human Technopole, Milan, Italy

    Carlos Jiménez & Nicola Crosetto

  2. Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden

    Nicola Crosetto

  3. Science for Life Laboratory, Solna, Sweden

    Nicola Crosetto

Authors
  1. Carlos Jiménez
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  2. Nicola Crosetto
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Correspondence to Nicola Crosetto.

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The authors declare no competing interests.

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Jiménez, C., Crosetto, N. Discovering CRISPR–Cas off-target breaks. Nat Methods 20, 641–642 (2023). https://doi.org/10.1038/s41592-023-01847-6

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