We evolved the brilliant monomeric red fluorescent protein mScarlet3 using a multiparameter screening approach. Owing to a newly engineered hydrophobic patch inside its β-barrel structure, mScarlet3 combines a high quantum yield and high fluorescence lifetime with fast and complete maturation. Consequently, mScarlet3 performs well as a fusion tag in live-cell imaging.
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This is a summary of: Gadella, T. W. J. Jr. et al. mScarlet3: a brilliant and fast-maturing red fluorescent protein. Nat. Methods https://doi.org/10.1038/s41592-023-01809-y (2023).
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A brilliant monomeric red fluorescent protein combining high brightness and fast maturation. Nat Methods 20, 497–498 (2023). https://doi.org/10.1038/s41592-023-01810-5
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DOI: https://doi.org/10.1038/s41592-023-01810-5
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