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Not if but when nanopore protein sequencing meets single-cell proteomics

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The nanopore community is stepping toward a new frontier of single-molecule protein sequencing. Here, we offer our opinions on the unique potential for this emerging technology, with a focus on single-cell proteomics, and some challenges that must be overcome to realize it.

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Fig. 1: Single-cell nanopore proteomics with highly multiplexable peptide sequence-based barcoding and highly parallel nanopore instruments.

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Acknowledgements

K.M. is supported by awards from the Japan Agency for Medical Research and Development (AMED) and the Japan Society for the Promotion of Science (JSPS). We thank Ying Zhu (Genentech) for feedback on this piece. We also thank BioRender for helping create the figure.

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Authors and Affiliations

  1. Paul G. Allen School of Computer Science and Engineering, University of Washington, Seattle, WA, USA

    Keisuke Motone & Jeff Nivala

  2. Molecular Engineering and Sciences Institute, University of Washington, Seattle, WA, USA

    Jeff Nivala

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  1. Keisuke Motone
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  2. Jeff Nivala
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Correspondence to Jeff Nivala.

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Competing interests

J.N. is a consultant to Oxford Nanopore Technologies. K.M. declares no competing interests.

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Motone, K., Nivala, J. Not if but when nanopore protein sequencing meets single-cell proteomics. Nat Methods 20, 336–338 (2023). https://doi.org/10.1038/s41592-023-01800-7

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