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Towards reproducibility in large-scale analysis of protein–protein interactions

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Co-fractionation mass spectrometry (CF-MS) has the potential to measure thousands of protein complexes in a single experiment, but the field is still in its infancy. A meta-analysis of CF-MS data yields a core CF-MS interactome and a tool allowing researchers to align new results to published data.

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Fig. 1: Three approaches for mapping protein–protein interactions.

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Acknowledgements

The authors thank Johanna Olweus for critical reading of the manuscript. F.L.-J. is partner in the National Network for Advance Proteomics Infrastructure (NAPI), which has received funding from the Norwegian Research Council.

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Authors and Affiliations

  1. Department of Immunology, Oslo University Hospital, Oslo, Norway

    Fridtjof Lund-Johansen, Trung Tran & Adi Mehta

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  1. Fridtjof Lund-Johansen
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  2. Trung Tran
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  3. Adi Mehta
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Correspondence to Fridtjof Lund-Johansen.

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The authors declare no competing interests.

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Lund-Johansen, F., Tran, T. & Mehta, A. Towards reproducibility in large-scale analysis of protein–protein interactions. Nat Methods 18, 720–721 (2021). https://doi.org/10.1038/s41592-021-01202-7

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