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Spatially resolved transcriptomics adds a new dimension to genomics

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As single-cell omics continue to advance, the field of spatially resolved transcriptomics has emerged with a set of experimental and computational methods to map out the positions of cells and their gene expression profiles in space. Here we summarize current transcriptome-wide and sequencing-based methodologies and their applications in genomics research.

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Fig. 1: Experimental and computational methods for sequencing-based spatial transcriptomics.
Fig. 2

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Authors and Affiliations

  1. Science for Life Laboratory, Department of Gene Technology, KTH Royal Institute of Technology, Stockholm, Sweden

    Ludvig Larsson & Joakim Lundeberg

  2. Department of Cell and Molecular Biology, Karolinska Institute, Stockholm, Sweden

    Jonas Frisén

Authors
  1. Ludvig Larsson
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  2. Jonas Frisén
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  3. Joakim Lundeberg
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Contributions

L.L. and J.L. wrote the manuscript with input from J.F.

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Correspondence to Joakim Lundeberg.

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Competing interests

J.L., J.F. and L.L. are scientific consultants to 10x Genomics Inc.

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Larsson, L., Frisén, J. & Lundeberg, J. Spatially resolved transcriptomics adds a new dimension to genomics. Nat Methods 18, 15–18 (2021). https://doi.org/10.1038/s41592-020-01038-7

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