A second-timescale optogenetic strategy DeKinomics was developed, enabling the study of downstream events of kinases in a gain-of-function manner. Using this technique, UBA1, an E1 enzyme that initiates the ubiquitination cascade, was found to be directly regulated by tyrosine phosphorylation.
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Chu, G., Li, YM. Lighting up kinase contacts in situ. Nat Chem Biol 20, 544–545 (2024). https://doi.org/10.1038/s41589-024-01543-2
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DOI: https://doi.org/10.1038/s41589-024-01543-2
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