This study establishes in vitro and in vivo systems to study mutagenesis and cancer genome evolution. Using microfluidics and mouse models, the method enables the dissection of co-occurring mutational processes and reveals that acute damage results in mirror-image mutation phasing between sister cells after a single mitosis.
This is a preview of subscription content, log in via an institution to check access.
References
The ICGC/TCGA Pan-Cancer Analysis of Whole Genomes Consortium. Pan-cancer analysis of whole genomes. Nature 578, 82–93 (2020). This paper reports whole-genome sequencing of 2,658 tumors.
Aitken, S. J. et al. Pervasive lesion segregation shapes cancer genome evolution. Nature 583, 265–270 (2020). This paper first describes the lesion segregation model.
Koh, S.-B. et al. A quantitative FastFUCCI assay defines cell cycle dynamics at a single-cell level. J. Cell Sci. 130, 512–520 (2017). This paper describes a variant of the fluorescence ubiquitination-based cell cycle indicator (FUCCI) cell cycle reporter.
Additional information
Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
This is a summary of: Ginno, P. A. et al. Single-mitosis dissection of acute and chronic DNA mutagenesis and repair. Nat. Genet. https://doi.org/10.1038/s41588-024-01712-y (2024).
Rights and permissions
About this article
Cite this article
Finding the lost sister: mirror-image mutation phasing in mammalian mitotic sister cells. Nat Genet 56, 746–747 (2024). https://doi.org/10.1038/s41588-024-01713-x
Published:
Issue Date:
DOI: https://doi.org/10.1038/s41588-024-01713-x
- Springer Nature America, Inc.