The underrepresentation of functional glial cells is a major challenge in brain organoid models. We developed an astroglia-enriched cortical organoid model that allows efficient generation of functional astrocytes and enables the formation of astroglial morphological subclasses with layer-specific gene expression profiles upon transplantation into the mouse brain.
This is a preview of subscription content, log in via an institution to check access.
References
Kim, J., Koo, B. K. & Knoblich, J. A. Human organoids: model systems for human biology and medicine. Nat. Rev. Mol. Cell Biol. 21, 571–584 (2020). A review article that presents human organoid models.
Freeman, M. R. Specification and morphogenesis of astrocytes. Science 330, 774–778 (2010). A review article that presents astrocyte development.
Mansour, A. et al. An in vivo model of functional and vascularized human brain organoids. Nat. Biotechnol. 36, 432–441 (2018). This paper reports an in vivo model of human brain organoids.
Oberheim, N. A. et al. Uniquely hominid features of adult human astrocytes. J. Neurosci. 29, 3276–3287 (2009). This paper reports unique features of adult human astrocytes.
Schafer, S. T. et al. An in vivo neuroimmune organoid model to study human microglia phenotypes. Cell 186, 2111–2126 (2023). This paper reports an in vivo cortical organoid model with human microglia.
Additional information
Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
This is a summary of: Wang, M. et al. Morphological diversification and functional maturation of human astrocytes in glia-enriched cortical organoid transplanted in mouse brain. Nat. Biotechnol., https://doi.org/10.1038/s41587-024-02157-8 (2024).
Rights and permissions
About this article
Cite this article
Glia-enriched cortical organoids implanted in mice capture astrocyte diversity. Nat Biotechnol (2024). https://doi.org/10.1038/s41587-024-02198-z
Published:
DOI: https://doi.org/10.1038/s41587-024-02198-z
- Springer Nature America, Inc.