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Generation of programmable splicing factors using RNA-binding proteins that activate exon inclusion

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We developed luciferase-based splicing reporters to evaluate 718 RNA-binding proteins (RBPs) for the ability to activate exon inclusion. Our screen detected RBPs with no previously characterized roles in splicing and utilized RBP domains displaying potent exon activation to develop programmable splicing modulators with improved strength, generalizability and size over previous versions.

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Fig. 1: Reporter design and validation.

References

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This is a summary of: Schmok, J. C. et al. Large-scale evaluation of the ability of RNA-binding proteins to activate exon inclusion. Nat. Biotechnol. https://doi.org/10.1038/s41587-023-02014-0 (2024).

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Generation of programmable splicing factors using RNA-binding proteins that activate exon inclusion. Nat Biotechnol (2024). https://doi.org/10.1038/s41587-023-02032-y

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  • DOI: https://doi.org/10.1038/s41587-023-02032-y

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