Wild three-dimensional imaging of solvent-cleared organs (wildDISCO) uses conventional antibodies for whole-body staining in mice, creating comprehensive biological atlases of nerves, blood vessels and lymphatics. It uncovers pathological changes, such as tertiary lymphoid structures in cancer, and it enables the precise tracking of therapeutic molecules and cells, enhancing our understanding of disease pathology and treatment.
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This is a summary of: Mai, H. et al. Whole-body cellular mapping in mouse using standard IgG antibodies. Nat. Biotechnol. https://doi.org/10.1038/s41587-023-01846-0 (2023).
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Breaking boundaries in whole-body imaging and disease understanding with wildDISCO. Nat Biotechnol 42, 576–577 (2024). https://doi.org/10.1038/s41587-023-01864-y
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DOI: https://doi.org/10.1038/s41587-023-01864-y
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