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CRISPR-free, strand-selective mitochondrial DNA base editing using a nickase

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The fusion of a programmable transcription-activator-like effector (TALE) protein with a nickase, in conjunction with a deaminase, enables efficient and strand-selective DNA base editing. This approach has the potential to advance our understanding and treatment of diseases associated with mutations in the mitochondrial or nuclear genome.

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Fig. 1: CRISPR-free, strand-selective DNA base editing with the introduction of a nickase.

References

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This is a summary of: Yi, Z. et al. Strand-selective base editing of human mitochondrial DNA using mitoBEs. Nat. Biotechnol. https://doi.org/10.1038/s41587-023-01791-y (2023).

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CRISPR-free, strand-selective mitochondrial DNA base editing using a nickase. Nat Biotechnol 42, 392–393 (2024). https://doi.org/10.1038/s41587-023-01820-w

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  • DOI: https://doi.org/10.1038/s41587-023-01820-w

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