Development is orchestrated in part by myriad chromatin-associated proteins that decorate the genome and physically coordinate in ways that are often unclear. An epigenomics technique called MulTI-Tag maps the genomic locations of different chromatin proteins in the same individual cells, helping to unveil their interactions.
This is a preview of subscription content, log in via an institution to check access.
References
Kaya-Okur, H. S. et al. CUT&Tag for efficient epigenomic profiling of small samples and single cells. Nat. Protoc. 10, 1930 (2019). Describes CUT&Tag, an antibody-guided Tn5 tagmentation-based epigenome profiling technique.
Bernstein, B. E. et al. A bivalent chromatin structure marks key developmental genes in embryonic stem cells. Cell 125, 315–326 (2006). Reports the first observation of ‘bivalent’ chromatin characterized by active (H3K4me3) and repressive (H3K27me3) histone modifications co-enriched in the same genes.
Additional information
Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
This is a summary of: Meers, M. P., Llagas, G., Janssens, D. H., Codomo, C. A. & Henikoff, S. Multifactorial profiling of epigenetic landscapes at single-cell resolution using MulTI-Tag. Nat. Biotechnol. https://doi.org/10.1038/s41587-022-01522-9 (2022)
Rights and permissions
About this article
Cite this article
Profiling chromatin–protein crosstalk at locus resolution in single cells. Nat Biotechnol 41, 624–625 (2023). https://doi.org/10.1038/s41587-022-01557-y
Published:
Issue Date:
DOI: https://doi.org/10.1038/s41587-022-01557-y
- Springer Nature America, Inc.