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Locus-specific chromatin isolation

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A Publisher Correction to this article was published on 03 February 2020

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Identifying factors bound to specific genomic loci in an unbiased manner is the holy grail of chromatin research, because it would provide an ultimate description of locus function. In this Comment, we explain why purifying a single locus represents a major technical challenge and provide recommendations for achieving this goal.

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References

  1. Gauchier, M. et al. Purification and enrichment of locus-specific proteomes: challenges and possibilities. Nat. Methods (in the press) (2020).

  2. Anderson, N. L. & Anderson, N. G. The human plasma proteome: history, character, and diagnostic prospects. Mol. Cell. Proteomics 1, 845–867 (2002).

    Article  CAS  Google Scholar 

  3. Déjardin, J. & Kingston, R. E. Purification of proteins associated with specific genomic loci. Cell 136, 175–186 (2009).

    Article  Google Scholar 

  4. Pourfarzad, F. et al. Locus-specific proteomics by TChP: targeted chromatin purification. Cell Rep. 4, 589–600 (2013).

    Article  CAS  Google Scholar 

  5. Ide, S. & Dejardin, J. End-targeting proteomics of isolated chromatin segments of a mammalian ribosomal RNA gene promoter. Nat. Commun. 6, 6674 (2015).

    Article  CAS  Google Scholar 

  6. Buxton, K. E. et al. Elucidating protein-DNA interactions in human alphoid chromatin via hybridization capture and mass spectrometry. J. Proteome Res. 16, 3433–3442 (2017).

    Article  CAS  Google Scholar 

  7. Liu, X. et al. In situ capture of chromatin interactions by biotinylated dCas9. Cell 170, 1028–1043 (2017).

    Article  CAS  Google Scholar 

  8. Tsui, C. et al. dCas9-targeted locus-specific protein isolation method identifies histone gene regulators. Proc. Natl Acad. Sci. USA 115, 201718844 (2018).

    Article  Google Scholar 

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Acknowledgements

Work in the laboratory of J. Dejardin is supported by grants from ARC Equipe Labélisée 2016, the ERC CoG METACHROM, from INCA, from the Fondation Schlumberger pour l’Education et la Recherche and from Merck (MSD). The laboratory of M. Vermeulen is part of the Oncode Institute, which is partly funded by the Dutch Cancer Society.

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Authors and Affiliations

  1. Department of Molecular Biology, Radboud Institute for Molecular Life Sciences, Oncode Institute, Radboud University, Nijmegen, The Netherlands

    Michiel Vermeulen

  2. Institute of Human Genetics, CNRS-Université de Montpellier, Montpellier, France

    Jérôme Déjardin

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  1. Michiel Vermeulen
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  2. Jérôme Déjardin
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Correspondence to Michiel Vermeulen or Jérôme Déjardin.

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The authors declare no competing interests.

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Vermeulen, M., Déjardin, J. Locus-specific chromatin isolation. Nat Rev Mol Cell Biol 21, 249–250 (2020). https://doi.org/10.1038/s41580-020-0217-0

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