Skip to main content
SpringerLink
Log in

Membrane nanodiscs/single-molecule analysis

Lightly counting membrane proteins in native nanodiscs

  • News & Views
  • Published:

From Nature Nanotechnology

View current issue Submit your manuscript

To determine the physiologically relevant oligomeric form of membrane proteins is extremely challenging. Now an elegant method of counting the oligomers in membrane proteins in near-native states is presented, using photobleaching and nanodiscs formed directly from cellular membranes.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1: Protein counting using the Native-nanoBleach method.

References

  1. Mulkidjanian, A. Y., Galperin, M. Y. & Koonin, E. V. Trends Biochem. Sci. 34, 206–215 (2009).

    Article  CAS  Google Scholar 

  2. Borcik, C. G. et al. J. Am. Chem. Soc. 142, 14102–14116 (2020).

    Article  CAS  Google Scholar 

  3. Chadda, R. et al. The dimerization equilibrium of a ClC Cl(-)/H(+) antiporter in lipid bilayers. eLife 5, e17438 (2016).

    Article  Google Scholar 

  4. Walker, G. et al. Nat. Nanotechnol. https://doi.org/10.1038/s41565-023-01547-4 (2023).

  5. Gwosch, K. C. et al. Nat. Methods 17, 217–224 (2020).

    Article  CAS  Google Scholar 

  6. Knowles, T. J. et al. J. Am. Chem. Soc. 131, 7484–7485 (2009).

    Article  CAS  Google Scholar 

  7. Ulbrich, M. H. & Isacoff, E. Y. Nat. Methods 4, 319–321 (2007).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Chemistry, The University of Chicago, Chicago, IL, USA

    Koushambi Mitra & Yamuna Krishnan

  2. Neuroscience Institute, The University of Chicago, Chicago, IL, USA

    Koushambi Mitra & Yamuna Krishnan

  3. Institute of Biophysical Dynamics, The University of Chicago, Chicago, IL, USA

    Koushambi Mitra & Yamuna Krishnan

Authors
  1. Koushambi Mitra
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yamuna Krishnan
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Yamuna Krishnan.

Ethics declarations

Competing interests

The authors declare no competing interests.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Mitra, K., Krishnan, Y. Lightly counting membrane proteins in native nanodiscs. Nat. Nanotechnol. 19, 5–6 (2024). https://doi.org/10.1038/s41565-023-01538-5

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/s41565-023-01538-5

  • Springer Nature Limited

Search

Navigation