We developed a high-throughput, unbiased strategy for the identification of endogenous biomolecular condensates by merging cell volume compression, sucrose density gradient centrifugation and quantitative mass spectrometry. We demonstrated the performance of this strategy by identifying both global condensate proteins and those responding to specific biological processes on a proteome-wide scale.
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This is a summary of: Li, P. et al. High-throughput and proteome-wide discovery of endogenous biomolecular condensates. Nat. Chem. https://doi.org/10.1038/s41557-024-01485-1 (2024).
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Identifying phase-separating biomolecular condensates in cells. Nat. Chem. (2024). https://doi.org/10.1038/s41557-024-01503-2
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DOI: https://doi.org/10.1038/s41557-024-01503-2
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