Multiple methods for deriving human cortical organoids have been established in the past decade. A study now systematically compares patterning strategies and shows that combined WNT and dual SMAD inhibition is superior to dual SMAD inhibition alone in inducing robust cortical identity in 3D human pluripotent stem-cell aggregates.
References
Del Dosso, A., Urenda, J. P., Nguyen, T. & Quadrato, G. Neuron 107, 1014–1028 (2020).
Atamian, A., Cordón-Barris, L. & Quadrato, G. Semin. Cell Dev. Biol. 111, 23–31 (2021).
Rosebrock, D. et al. Nat. Cell Biol. https://doi.org/10.1038/s41556-022-00929-5 (2022).
Hansen, D. V., Lui, J. H., Parker, P. R. L. & Kriegstein, A. R. Nature 464, 554–561 (2010).
Pollen, A. A. et al. Cell 163, 55–67 (2015).
Watanabe, K. et al. Nat. Neurosci. 8, 288–296 (2005).
Eiraku, M. et al. Cell Stem Cell 3, 519–532 (2008).
Elkabetz, Y. et al. Genes Dev. 22, 152–165 (2008).
Chambers, S. M. et al. Nat. Biotechnol. 27, 275–280 (2009).
Shalem, O. et al. Science 343, 84 (2014).
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Competing interests
The authors declare no competing interests.
Rights and permissions
About this article
Cite this article
Atamian, A., Birtele, M. & Quadrato, G. Not all cortical organoids are created equal. Nat Cell Biol 24, 805–806 (2022). https://doi.org/10.1038/s41556-022-00890-3
Published:
Issue Date:
DOI: https://doi.org/10.1038/s41556-022-00890-3
- Springer Nature Limited