CRISPR–Cas12a was used to directly replace mouse antibody variable chain genes with human versions in primary B cells. The edited cells underwent affinity maturation in vivo, improving the potency of HIV-1 and SARS-CoV-2 neutralizing antibodies without loss of bioavailability. Affinity maturation of edited cells also enables new vaccine models and adaptive B cell therapies.
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References
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This is a summary of: Yin, Y. et al. In vivo affinity maturation of mouse B cells reprogrammed to express human antibodies. Nat. Biomed. Eng. https://doi.org/10.1038/s41551-024-01179-6 (2024).
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Affinity maturation of CRISPR-engineered B cell receptors in vivo. Nat. Biomed. Eng 8, 341–342 (2024). https://doi.org/10.1038/s41551-024-01184-9
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DOI: https://doi.org/10.1038/s41551-024-01184-9
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