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Probing when dCas9 tolerates DNA mismatches

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Sensing changes in ionic current as barcoded DNA translocates through solid-state nanopores allows the study of how nucleotide sequences alter the DNA-binding specificity of the catalytically inactive Cas9 ribonucleoprotein complex.

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Fig. 1: Assessing the binding specificity of dCas9 to target sequences via barcoded DNA translocating through a solid-state nanopore.

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  1. Departments of Electrical Engineering and Biomedical Engineering, Pennsylvania State University, University Park, PA, USA

    Weihua Guan

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  1. Weihua Guan
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Correspondence to Weihua Guan.

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Guan, W. Probing when dCas9 tolerates DNA mismatches. Nat. Biomed. Eng 8, 207–208 (2024). https://doi.org/10.1038/s41551-023-01172-5

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