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Multipass high-dimensional flow cytometry

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Barcoding cells with microparticles that emit near-infrared laser light enables the use of flow cytometry to track the dynamics of single cells by using more markers and fewer colours.

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Fig. 1: High-dimensional multipass flow cytometry via barcoded cells.

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Acknowledgements

This work was supported by the grant Giovani Ricercatori GR-2016-02363315 (to E.L.) from the Italian Ministry of Health, and by the Intramural Research Program of the Vaccine Research Center, NIAID, NIH (to M.R.).

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Authors and Affiliations

  1. Laboratory of Translational Immunology, IRCCS Humanitas Research Hospital, Milan, Italy

    Enrico Lugli & Rosa Sottile

  2. ImmunoTechnology Section, Vaccine Research Center, NIAID, NIH, Bethesda, MD, USA

    Mario Roederer

Authors
  1. Enrico Lugli
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  2. Mario Roederer
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  3. Rosa Sottile
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Correspondence to Enrico Lugli.

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Competing interests

E.L. serves as a consultant for BD Biosciences. The other authors declare no competing interests.

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Lugli, E., Roederer, M. & Sottile, R. Multipass high-dimensional flow cytometry. Nat. Biomed. Eng 8, 209–211 (2024). https://doi.org/10.1038/s41551-023-01165-4

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