An efficient protocol for the preparation of DNA libraries for the analysis of methylation patterns in cell-free DNA in plasma enhances the sensitivity of bisulfite sequencing for the early detection of lung cancer.
This is a preview of subscription content, log in via an institution to check access.
References
Heitzer, E. et al. Nat. Rev. Genet. 20, 71–88 (2019).
Liu, M. C. et al. Ann. Oncol. 31, 745–759 (2020).
Parikh, A. R. et al. Clin. Cancer Res. https://doi.org/10.1158/1078-0432.CCR-21-0410 (2021).
Liang, N. et al. Nat. Biomed. Eng. https://doi.org/10.1038/s41551-021-00746-5 (2021).
Gansauge, M.-T. & Meyer, M. Nat. Protoc. 8, 737–748 (2013).
Schumacher, C., Kurihara, L. & Cunningham, K. Nat. Meth. 12, v–vi (2015).
The Cancer Genome Atlas Research Network et al. Nat. Genet. 45, 1113–1120 (2013).
Guo, S. et al. Nat. Genet. 49, 635–642 (2017).
Liu, X. et al. EBioMedicine 41, 345–356 (2019).
Liu, Y. et al. Nat. Biotechnol. 37, 424–429 (2019).
Vaisvila, R. et al. Preprint at https://www.biorxiv.org/content/10.1101/2019.12.20.884692v2 (2019).
Schutsky, E. K. et al. Nat. Biotechnol. 36, 1083–1090 (2018).
Shen, S. Y., Burgener, J. M., Bratman, S. V. & De Carvalho, D. D. Nat. Protoc. 14, 2749–2780 (2019).
Shen, S. Y. et al. Nature 563, 579–583 (2018).
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Competing interests
I.S.H. is a former employee of Freenome and a shareholder in the company.
Rights and permissions
About this article
Cite this article
Haque, I.S. Enhanced DNA libraries for methylation analysis. Nat Biomed Eng 5, 490–492 (2021). https://doi.org/10.1038/s41551-021-00750-9
Published:
Issue Date:
DOI: https://doi.org/10.1038/s41551-021-00750-9
- Springer Nature Limited