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Orthotopic editing of T-cell receptors

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The simultaneous removal of endogenous T-cell receptor α-chains and β-chains, and the orthotopic placement of an exogenous receptor in human T cells via CRISPR gene-editing, prevents the mispairing between endogenous and transgenic receptors while preserving the cells’ function.

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Fig. 1: T-cell engineering for the generation of therapeutic T cells.

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Authors and Affiliations

  1. Laboratory of Tumor Immunology, Department of Medical Oncology, Erasmus MC-Cancer Institute, Rotterdam, The Netherlands

    Dian Kortleve, Dora Hammerl & Reno Debets

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  1. Dian Kortleve
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  2. Dora Hammerl
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  3. Reno Debets
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Correspondence to Reno Debets.

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Kortleve, D., Hammerl, D. & Debets, R. Orthotopic editing of T-cell receptors. Nat Biomed Eng 3, 949–950 (2019). https://doi.org/10.1038/s41551-019-0490-4

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