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Powered and controlled T-cell production

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T cells can be efficiently and controllably expanded for adoptive cell therapy in a culture system consisting of lipid bilayers bearing membrane-bound cues for the stimulation of T-cell receptors and of supporting silica microrods that release a T-cell proliferation factor.

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Fig. 1: A scaffold for the efficient ex vivo expansion of T cells.
Fig. 2: Overview of the ex vivo manufacturing process of chimeric antigen receptor T (CAR-T) cells.

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Authors and Affiliations

  1. Center for Cellular Immunotherapies and Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, PA, USA

    Michael C. Milone & Bruce L. Levine

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  1. Michael C. Milone
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  2. Bruce L. Levine
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Correspondence to Bruce L. Levine.

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Competing interests

M.C.M. and B.L.L. have commercial research grants from Novartis Pharmaceuticals, have ownership interest in intellectual property and patents held by the University of Pennsylvania, and are co-founders of Tycho Therapeutics (M.C.M.) and Tmunity Therapeutics (B.L.L.). These relationships are managed in accordance with University of Pennsylvania policies and oversight.

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Milone, M.C., Levine, B.L. Powered and controlled T-cell production. Nat Biomed Eng 2, 148–150 (2018). https://doi.org/10.1038/s41551-018-0206-1

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