Skip to main content
SpringerLink
Log in

An all-in-one targeted protein degradation platform guided by degradation condensates-bridging bi-specific nanobodies

  • Letter to the Editor
  • Published:
Cell Research Submit manuscript

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1: Construction of the general PDF-Bin platform.

References

  1. Pettersson, M. & Crews, C. M. Drug Discov. Today Technol. 31, 15–27 (2019).

    Article  PubMed  PubMed Central  Google Scholar 

  2. Banik, S. M. et al. Nature 584, 291–297 (2020).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Takahashi, D. et al. Mol. Cell 76, 797–810.e10 (2019).

    Article  CAS  PubMed  Google Scholar 

  4. Li, Z. et al. Nature 575, 203–209 (2019).

    Article  CAS  PubMed  Google Scholar 

  5. Ji, C. H. et al. Nat. Commun. 13, 904 (2022).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Sun, D., Wu, R., Zheng, J., Li, P. & Yu, L. Cell Res. 28, 405–415 (2018).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Fu, A., Cohen-Kaplan, V., Avni, N., Livneh, I. & Ciechanover, A. Proc. Natl. Acad. Sci. USA 118, e2107321118 (2021).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Moutel, S. et al. Elife 5, e16228 (2016).

    Article  PubMed  PubMed Central  Google Scholar 

  9. Xu, W. et al. Cell Rep. 36, 109482 (2021).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Kubala, M. H., Kovtun, O., Alexandrov, K. & Collins, B. M. Protein Sci. 19, 2389–2401 (2010).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Feng, X. et al. Cell Res. 32, 659–669 (2022).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Yang, Y. et al. Nat. Commun. 10, 3759 (2019).

    Article  PubMed  PubMed Central  Google Scholar 

  13. Zhao, C. et al. Trends Pharmacol. Sci. 37, 47–61 (2016).

    Article  PubMed  Google Scholar 

  14. La Sala, G. et al. Nat. Commun. 11, 4115 (2020).

    Article  PubMed  PubMed Central  Google Scholar 

  15. Fridy, P. C. et al. Nat. Methods 11, 1253–1260 (2014).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

Download references

Acknowledgements

This work was supported by grants from the National Natural Science Foundation of China (32150023 to P.L.), the National Key R&D Program (2019YFA0508403 and 2023YFF1204703 to P.L.), the Natural Science Foundation of Xiamen, China (3502Z20227251 to Z.L.), Xiamen Ocean and Fisheries Development Special Funds Program (22CZP006HJ03 to Z.L.).

Author information

Authors and Affiliations

  1. State Key Laboratory of Membrane Biology & Frontier Research Center for Biological Structure, School of Life Sciences, Tsinghua University, Tsinghua University-Peking University Joint Center for Life Sciences, Beijing, China

    Wen Jia & Pilong Li

  2. Key Laboratory of Marine Genetic Resources, State Key Laboratory Breeding Base of Marine Genetic Resources, Fujian Key Laboratory of Marine Genetic Resources, Fujian Collaborative Innovation Centre for Exploitation and Utilization of Marine Biological Resources, Third Institute of Oceanography Ministry of Natural Resources, Xiamen, Fujian, China

    Weijie Li & Zengpeng Li

Authors
  1. Wen Jia
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Weijie Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Zengpeng Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Pilong Li
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

P.L conceived the ideas. Z.L. and P.L supervised the project. W.J. performed cell biology and biochemical experiments. W.L. performed the selection of nanobody and affinity assay. W.J. wrote the manuscript. Z.L. and P.L. edited the manuscript. All authors have commented on and approved the manuscript.

Corresponding authors

Correspondence to Zengpeng Li or Pilong Li.

Ethics declarations

Competing interests

P.L. is the scientific co-founder of NuPhase Therapeutics, whose projects are not related to this work. Other authors declare that they have no other conflict of interest.

Supplementary information

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Jia, W., Li, W., Li, Z. et al. An all-in-one targeted protein degradation platform guided by degradation condensates-bridging bi-specific nanobodies. Cell Res 34, 389–392 (2024). https://doi.org/10.1038/s41422-024-00942-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/s41422-024-00942-3

  • Springer Nature Singapore Pte Ltd.

Search

Navigation