This is a preview of subscription content, log in via an institution to check access.
Data availability
The electron density maps and the atomic coordinates for F8–A22–E4 machinery in apo (without DNA), with no visible DNA, and with DNA were deposited in Electron Microscopy Data Bank and Protein Data Bank with accession codes EMD-34684 and 8HDZ, EMD-34927 and 8HOY, and EMD-34929 and 8HPA, respectively.
References
Isidro, J. et al. Nat. Med. 28, 1569–1572 (2022).
Endo, A. et al. Science 378, 90–94 (2022).
Czarnecki, M. W. & Traktman, P. Virus Res. 234, 193–206 (2017).
Stanitsa, E. S., Arps, L. & Traktman, P. J. Biol. Chem. 281, 3439–3451 (2006).
Boyle, K. A. et al. J. Biol. Chem. 286, 24702–24713 (2011).
Siegrist, E. A. & Sassine, J. Clin. Infect. Dis. 76, 155–164 (2023).
Tarbouriech, N. et al. Nat. Commun. 8, 1455 (2017).
Bersch, B., Tarbouriech, N., Burmeister, W. P. & Iseni, F. J. Mol. Biol. 433, 167009 (2021).
Jain, R. et al. Nat. Struct. Mol. Biol. 26, 955–962 (2019).
Hostetler, K. Y. Antiviral Res. 82, A84–A98 (2009).
Trost, L. C. et al. Antiviral Res. 117, 115–121 (2015).
Wang, M. et al. Biochemistry 50, 581–590 (2011).
Killelea, T. et al. Biochemistry 49, 5772–5781 (2010).
Porecha, R. H. & Stivers, J. T. Proc. Natl. Acad. Sci. USA 105, 10791–10796 (2008).
Sele, C. et al. J. Virol. 87, 1679–1689 (2013).
Acknowledgements
We are grateful for the excellent support from Dr. Yi Zeng and all members of the cryo-EM center and the core facility of Wuhan University. We thank Thomas Wileman (University of East Anglia) for critical reading of the manuscript. We want to express our gratitude to all our team members for their supports and discussions. We thank financial supports from the National Natural Science Foundation of China (32250710142), and Strategy and Talent Support Program of Wuhan University.
Author information
Authors and Affiliations
Contributions
C.D. conceived, coordinated and supervised the study. Y.X., Y.Z., Y.W., R.F. and Y.Y. performed gene cloning, protein expression, purification, and cryo-EM sample preparation. Y.W., D.L., S.Z. and B.Y. performed cryo-EM sample screening and data collection. Z.Z., C.D. and Y.W. conduced data processing and model building. C.D., Z.Z., Y.X. and Y.W. wrote the manuscript. All authors reviewed and edited the manuscript.
Corresponding authors
Ethics declarations
Competing interests
The authors declare no competing interests.
Supplementary information
Rights and permissions
About this article
Cite this article
Xu, Y., Wu, Y., Zhang, Y. et al. Cryo-EM structures of human monkeypox viral replication complexes with and without DNA duplex. Cell Res 33, 479–482 (2023). https://doi.org/10.1038/s41422-023-00796-1
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/s41422-023-00796-1
- Springer Nature Singapore Pte Ltd.