Crystal structures of T4 RNA ligase 2 trapped at different stages of a multistep reaction show how one enzyme active site juggles three phosphoryl transfer reactions to join two RNA strands. The structures also show how the ligase enzyme selectively recognizes the sugar pucker of ribose to achieve catalytic specificity toward RNA-containing substrates.
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Pascal, J., Ellenberger, T. RNA ligase does the AMP shuffle. Nat Struct Mol Biol 13, 950–951 (2006). https://doi.org/10.1038/nsmb1106-950
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DOI: https://doi.org/10.1038/nsmb1106-950
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