A key to understanding bacterial pathogenicity is the mechanism by which water-soluble protein toxins assemble on cell membranes to form oligomeric bilayer-spanning pores. The recent reconstructions from cryo-electron micrographs of three-dimensional pore and prepore structures of the cholesterol-dependent toxin pneumolysin shed new light on the later steps of the assembly of large toxin pores.
References
Song, L. et al. Science 274, 1859–1865 (1996).
Ramachandran, R., Tweten, R.K. & Johnson, A.E. Nat. Struct. Mol. Biol. 11, 697–705 (2004).
Tilley, S.J., Orlova, E.V., Gilbert, R.J.C., Andrew, P.W. & Saibil, H.R. Cell 121, 247–256 (2005).
Rossjohn, J., Fell, S.C., McKinstry, W.J., Tweten, R.K. & Parker, M.W. Cell 89, 685–692 (1997).
Walker, B.J., Krishnasastry, M., Zorn, L. & Bayley, H. J. Biol. Chem. 267, 21782–21786 (1992).
Walker, B., Braha, O., Cheley, S. & Bayley, H. Chem. Biol. 2, 99–105 (1995).
van der Goot, F.G., Pattus, F., Wong, K.R. & Buckley, J.T. Biochemistry 32, 2636–2642 (1993).
Sellman, B.R., Kagan, B.L. & Tweten, R.K. Mol. Microbiol. 23, 551–558 (1997).
Miller, C.J., Elliott, J.L. & Collier, R.J. Biochemistry 38, 10432–10441 (1999).
Nguyen, T.V., Higuchi, H. & Kamio, Y. Mol. Microbiol. 45, 1485–1498 (2002).
Hotze, E.M. et al. J. Biol. Chem. 276, 8261–8268 (2001).
Olson, R., Nariya, H., Yokota, K., Kamio, Y. & Gouaux, E. Nat. Struct. Biol. 6, 134–140 (1999).
Pédelacq, J.-D. et al. Structure 7, 277–288 (1999).
Guillet, V. et al. J. Biol. Chem. 279, 41028–41037 (2004).
Fang, Y., Cheley, S., Bayley, H. & Yang, J. Biochemistry 36, 9518–9522 (1997).
Kawate, T. & Gouaux, E. Protein Sci. 12, 997–1006 (2003).
Melton, J.A., Parker, M.W., Rossjohn, J., Buckley, J.T. & Tweten, R.K. J. Biol. Chem. 279, 14315–14322 (2004).
Czajkowsky, D.M., Hotze, E.M., Shao, Z. & Tweten, R.K. EMBO J. 23, 3206–3215 (2004).
Olson, R. & Gouaux, E. Protein Sci. 12, 379–383 (2003).
Palmer, M. et al. EMBO J. 17, 1598–1605 (1998).
Nguyen, V.T., Kamio, Y. & Higuchi, H. EMBO J. 19, 4968–4979 (2003).
Tamm, L.K., Hong, H. & Liang, B. Biochim. Biophys. Acta 1666, 250–263 (2004).
Sellman, B.R., Mourez, M. & Collier, R.J. Science 292, 695–697 (2001).
Iacovache, I. & van der Goot, F.G. Nat. Struct. Mol. Biol. 11, 1163–1164 (2004).
Giddings, K.S., Johnson, A.E. & Tweten, R.K. Proc. Natl. Acad. Sci. USA 100, 11315–11320 (2003).
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Bayley, H., Jayasinghe, L. & Wallace, M. Prepore for a breakthrough. Nat Struct Mol Biol 12, 385–386 (2005). https://doi.org/10.1038/nsmb0505-385
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DOI: https://doi.org/10.1038/nsmb0505-385
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