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Mechanisms for targeted, purposeful mutation revealed in an APOBEC–DNA complex

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Targeted deamination of cytosine bases in DNA by AID/APOBEC-family enzymes is critical for proper immune function, but it also poses risks to genomic integrity. New structures reported by Harris, Aihara and colleagues offer the first glimpses into the enzyme–DNA complex and reveal both expected and unexpected insights into the DNA-binding mode involved in targeting purposeful mutation.

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Figure 1: A3A and A3B as genomic mutators.
Figure 2: A3A-DNA interactions.

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References

  1. Siriwardena, S.U., Chen, K. & Bhagwat, A.S. Chem. Rev. 116, 12688–12710 (2016).

    Article  CAS  Google Scholar 

  2. Nik-Zainal, S. et al. Cell 149, 979–993 (2012).

    Article  CAS  Google Scholar 

  3. Roberts, S.A. et al. Mol. Cell 46, 424–435 (2012).

    Article  CAS  Google Scholar 

  4. Roberts, S.A. et al. Nat. Genet. 45, 970–976 (2013).

    Article  CAS  Google Scholar 

  5. Burns, M.B. et al. Nature 494, 366–370 (2013).

    Article  CAS  Google Scholar 

  6. Chan, K. et al. Nat. Genet. 47, 1067–1072 (2015).

    Article  CAS  Google Scholar 

  7. Kohli, R.M. et al. J. Biol. Chem. 284, 22898–22904 (2009).

    Article  CAS  Google Scholar 

  8. Salter, J.D., Bennett, R.P. & Smith, H.C. Trends Biochem. Sci. 41, 578–594 (2016).

    Article  CAS  Google Scholar 

  9. Shi, K., Carpenter, M.A., Kurahashi, K., Harris, R.S. & Aihara, H. J. Biol. Chem. 290, 28120–28130 (2015).

    Article  CAS  Google Scholar 

  10. Byeon, I.J. et al. Nat. Commun. 4, 1890 (2013).

    Article  Google Scholar 

  11. Bohn, M.F. et al. Structure 23, 903–911 (2015).

    Article  CAS  Google Scholar 

  12. Bulliard, Y. et al. J. Virol. 85, 1765–1776 (2011).

    Article  CAS  Google Scholar 

  13. Shi, K. et al. Nat. Struct. Mol. Biol. 24, 131–139 (2016).

    Article  Google Scholar 

  14. Logue, E.C. et al. PLoS One 9, e97062 (2014).

    Article  Google Scholar 

  15. Mitra, M. et al. Nucleic Acids Res. 42, 1095–1110 (2014).

    Article  CAS  Google Scholar 

  16. Losey, H.C., Ruthenburg, A.J. & Verdine, G.L. Nat. Struct. Mol. Biol. 13, 153–159 (2006).

    Article  CAS  Google Scholar 

  17. Hong, S. & Cheng, X. Adv. Exp. Med. Biol. 945, 321–341 (2016).

    Article  CAS  Google Scholar 

  18. Kazanov, M.D. et al. Cell Rep. 13, 1103–1109 (2015).

    Article  CAS  Google Scholar 

  19. Green, A.M. et al. Cell Cycle 15, 998–1008 (2016).

    Article  CAS  Google Scholar 

  20. Maciejowski, J., Li, Y., Bosco, N., Campbell, P.J. & de Lange, T. Cell 163, 1641–1654 (2015).

    Article  CAS  Google Scholar 

  21. Komor, A.C., Badran, A.H. & Liu, D.R. Cell 168, 20–36 (2016).

    Article  Google Scholar 

Download references

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Authors and Affiliations

  1. Zachary M. Hostetler and Rahul M. Kohli are at the Department of Medicine and the Department of Biochemistry and Biophysics at the Perelman School of Medicine, Emily K. Schutsky, University of Pennsylvania, Philadelphia, Pennsylvania, USA.,

    Emily K Schutsky, Zachary M Hostetler & Rahul M Kohli

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  1. Emily K Schutsky
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  2. Zachary M Hostetler
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  3. Rahul M Kohli
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Correspondence to Rahul M Kohli.

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Schutsky, E., Hostetler, Z. & Kohli, R. Mechanisms for targeted, purposeful mutation revealed in an APOBEC–DNA complex. Nat Struct Mol Biol 24, 97–98 (2017). https://doi.org/10.1038/nsmb.3373

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