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Expanding the RNA-recognition code of PUF proteins

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Classical PUF proteins bind to single-stranded RNA with sequence specificity that can be engineered by site-directed mutagenesis according to a simple RNA-recognition code. Now in-depth probing of the PUF RNA-recognition code enhances future design of PUF proteins and exposes hidden complexity in generating specificity.

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Figure 1: RNA recognition by PUF proteins.

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Authors and Affiliations

  1. Traci M. Tanaka Hall is at the Laboratory of Structural Biology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina, USA.,

    Traci M Tanaka Hall

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  1. Traci M Tanaka Hall
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Correspondence to Traci M Tanaka Hall.

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Hall, T. Expanding the RNA-recognition code of PUF proteins. Nat Struct Mol Biol 21, 653–655 (2014). https://doi.org/10.1038/nsmb.2863

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