Abstract
Telomere shortening and disruption of telomeric components are pathways that induce telomere deprotection. Here we describe another pathway, in which prolonged mitotic arrest induces damage signals at telomeres in human cells. Exposure to microtubule drugs, kinesin inhibitors, proteasome inhibitors or the disruption of proper chromosome cohesion resulted in the formation of damage foci at telomeres. Induction of mitotic telomere deprotection coincided with dissociation of TRF2 from telomeres, telomeric 3′-overhang degradation and ATM activation, and deprotection could be suppressed by TRF2 overexpression or inhibition of Aurora B kinase. Normal cells that escaped from prolonged mitotic arrest halted in the following G1 phase, whereas cells lacking p53 continued to cycle and became aneuploid. We propose a telomere-dependent mitotic-duration monitoring system that reacts to improper progression through mitosis.
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Acknowledgements
We thank J.M. Peters (Institute for Molecular Pathology), T. de Lange (The Rockefeller University) and P. Baumann (Stowers Institute for Medical Research) for antibodies; D. Gibbs (The Salk Institute) for production of lentivirus; and the Karlseder Laboratory for comments. M.T.H. is supported by a Human Frontier Science Program Long Term Fellowship and a Japan Society for the Promotion of Science Postdoctoral Fellowship for Research Abroad. A.J.C. is supported by a training grant from the US National Institutes of Health (NIH) (5T32CA009370-29). This work was supported by the Salk Institute Cancer Center Core Grant P30 CA014195-38 and grants from the NIH to J.K. (AG025837, GM087476).
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M.T.H. designed and carried out experiments and wrote the manuscript, A.J.C. designed and carried out experiments and wrote the manuscript, J.A.J.F. provided imaging expertise, E.L.-D. designed and carried out experiments, and J.K. designed experiments and wrote the manuscript.
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Hayashi, M., Cesare, A., Fitzpatrick, J. et al. A telomere-dependent DNA damage checkpoint induced by prolonged mitotic arrest. Nat Struct Mol Biol 19, 387–394 (2012). https://doi.org/10.1038/nsmb.2245
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DOI: https://doi.org/10.1038/nsmb.2245
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