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Nonsense-mediated mRNA decapping occurs on polyribosomes in Saccharomyces cerevisiae

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Abstract

Nonsense-mediated decay (NMD) degrades mRNA containing premature translation termination codons. In yeast, NMD substrates are decapped and digested exonucleolytically from the 5′ end. Despite the requirement for translation in recognition, degradation of nonsense-containing mRNA is considered to occur in ribosome-free cytoplasmic P bodies. We show decapped nonsense-containing mRNA associate with polyribosomes, indicating that recognition and degradation are tightly coupled and that polyribosomes are major sites for degradation of aberrant mRNAs.

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Figure 1: NMD substrates are bound by polyribosomes when mRNA decapping is inhibited.
Figure 2: The association of decapped NMD substrates with polyribosomes.
Figure 3: Decapped products of nonsense-containing mRNA co-purify with ribosomes.

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Acknowledgements

The authors thank members of the Baker and Coller laboratories for critical evaluation of the manuscript. W.H. is supported by the American Heart Association. C.P. is supported by an undergraduate stipend provided by the US National Science Foundation. Funding was provided by the US National Institutes of Health (GM080465).

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Authors and Affiliations

  1. Center for RNA Molecular Biology, Case Western Reserve University, Cleveland, Ohio, USA

    Wenqian Hu, Christine Petzold, Jeff Coller & Kristian E Baker

Authors
  1. Wenqian Hu
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  2. Christine Petzold
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  3. Jeff Coller
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  4. Kristian E Baker
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Contributions

W.H., J.C. and K.E.B. designed experimental strategies; W.H. and C.P. performed experimental analysis and interpreted data; J.C. and K.E.B. interpreted data and wrote the manuscript.

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Correspondence to Jeff Coller or Kristian E Baker.

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The authors declare no competing financial interests.

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Supplementary Text and Figures

Supplenentary Figures 1–4, Supplementary Table 1 and Supplementary Data (PDF 3446 kb)

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Hu, W., Petzold, C., Coller, J. et al. Nonsense-mediated mRNA decapping occurs on polyribosomes in Saccharomyces cerevisiae. Nat Struct Mol Biol 17, 244–247 (2010). https://doi.org/10.1038/nsmb.1734

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  • DOI: https://doi.org/10.1038/nsmb.1734

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