Abstract
DNA methylation is an epigenetic mark affecting genes and transposons. Screening for mutants that fail to establish DNA methylation yielded two we termed “involved in de novo” (idn) 1 and 2. IDN1 encodes DMS3, an SMC-related protein, and IDN2 encodes a previously unknown double-stranded RNA–binding protein with homology to SGS3. IDN1 and IDN2 control de novo methylation and small interfering RNA (siRNA)-mediated maintenance methylation and are components of the RNA-directed DNA methylation pathway.
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Acknowledgements
We thank J. Goodrich and D. Schubert for kindly providing T-DNA homozygous lines. We thank members of the Jacobsen laboratory for supportive discussions. Jacobsen lab research was supported by US National Institutes of Health grant GM60398. I.A. was supported by a postdoctoral fellowship from the Ministerio de Educacion y Ciencia. S.E.J. and J.C. are investigators of the Howard Hughes Medical Institute.
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T.C.M. and J.C. provided the majority of the insertion mutagenized population. I.A. and S.E.J. designed the experiments. I.A. performed the experiments and wrote the manuscript.
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Supplementary Figures 1–3, Supplementary Tables 1 and 2 and Supplementary Methods (PDF 170 kb)
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Ausin, I., Mockler, T., Chory, J. et al. IDN1 and IDN2 are required for de novo DNA methylation in Arabidopsis thaliana. Nat Struct Mol Biol 16, 1325–1327 (2009). https://doi.org/10.1038/nsmb.1690
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DOI: https://doi.org/10.1038/nsmb.1690
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