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Localizing the NADP+ binding site on the MurB enzyme by NMR

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NMR resonances of backbone nuclei have been assigned for >95% of the residues in oxidized, substrate-free, perdeuterated 13C/15N-labelled MurB (347 residues). A novel approach utilizing minimum chemical shift changes was employed to localize the NADP+ binding site on MurB.

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Author notes

  1. Bennett T. Farmer and Keith L. Constantine: These two authors contributed equally.

Authors and Affiliations

  1. Pharmaceutical Research Institute, Bristol-Myers Squibb Co., P.O. Box 4000, Princeton, New Jersey, 08543-4000, USA

    Bennett T. Farmer II, Keith L. Constantine, Valentina Goldfarb, Mark S. Friedrichs, Michael Wittekind, Joseph Yanchunas Jr., James G. Robertson & Luciano Mueller

Authors
  1. Bennett T. Farmer II
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  2. Keith L. Constantine
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  3. Valentina Goldfarb
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  4. Mark S. Friedrichs
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  5. Michael Wittekind
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  6. Joseph Yanchunas Jr.
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  7. James G. Robertson
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  8. Luciano Mueller
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Farmer, B., Constantine, K., Goldfarb, V. et al. Localizing the NADP+ binding site on the MurB enzyme by NMR. Nat Struct Mol Biol 3, 995–997 (1996). https://doi.org/10.1038/nsb1296-995

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