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Microtiter plate transformation using the LiAc/SS carrier DNA/PEG method

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Abstract

Here, we describe a protocol that has been adapted for the transformation of yeast cells in 96-well microtiter plates. This protocol can be tailored for multiple applications and is suitable for high-throughput applications. It can be completed in 2–3 h, once the yeast cells have been grown depending on the heat shock used.

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Change history

  • 04 December 2008

    In the version of this article initially published, in the Reagent Setup section on p. 6, the recipe for lithium acetate (1.0 M) called for 102 g of lithium acetate dihydrate. This should be 10.2 g. The error has been corrected in the HTML and PDF versions of the article.

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Authors and Affiliations

  1. Department of Biochemistry and Medical Genetics, University of Manitoba, T250-770 Bannatyne Ave., Winnipeg, R3E 0W3, Manitoba, Canada

    R Daniel Gietz

  2. Department of Pathology, Environmental Health and Radiation Oncology, UCLA School of Public Health and David Geffen School of Medicine, 650 Charles E. Young Drive South, Los Angeles, 90095, California, USA

    Robert H Schiestl

Authors
  1. R Daniel Gietz
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  2. Robert H Schiestl
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Correspondence to Robert H Schiestl.

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The authors declare no competing financial interests.

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Gietz, R., Schiestl, R. Microtiter plate transformation using the LiAc/SS carrier DNA/PEG method. Nat Protoc 2, 5–8 (2007). https://doi.org/10.1038/nprot.2007.16

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