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High-efficiency yeast transformation using the LiAc/SS carrier DNA/PEG method

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Abstract

Here we describe a high-efficiency version of the lithium acetate/single-stranded carrier DNA/PEG method of transformation of Saccharomyces cerevisiae. This method currently gives the highest efficiency and yield of transformants, although a faster protocol is available for small number of transformations. The procedure takes up to 1.5 h, depending on the length of heat shock, once the yeast culture has been grown. This method is useful for most transformation requirements.

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Change history

  • 20 November 2008

    Note: In the version of this article initially published, in the Reagent Setup section on p. 32, the recipe for lithium acetate (1.0 M) called for 102 g of lithium acetate dihydrate. This should be 10.2 g. The error has been corrected in the HTML and PDF versions of the article.

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Authors and Affiliations

  1. Department of Biochemistry and Medical Genetics, University of Manitoba, T250-770 Bannatyne Ave., Winnipeg, R3E 0W3, Manitoba, Canada

    R Daniel Gietz

  2. Department of Pathology, Environmental Health and Radiation Oncology, UCLA School of Public Health and David Geffen School of Medicine, 650 Charles E. Young Drive South, Los Angeles, 90095, California, USA

    Robert H Schiestl

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  1. R Daniel Gietz
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  2. Robert H Schiestl
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Correspondence to Robert H Schiestl.

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Gietz, R., Schiestl, R. High-efficiency yeast transformation using the LiAc/SS carrier DNA/PEG method. Nat Protoc 2, 31–34 (2007). https://doi.org/10.1038/nprot.2007.13

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