Abstract
Parvalbumin-expressing, fast-spiking basket cells are important for the generation of synchronous, rhythmic population activities in the hippocampus. We found that GABAA receptor–mediated synaptic inputs from murine parvalbumin-expressing basket cells were selectively modulated by the membrane voltage– and intracellular chloride–dependent chloride channel ClC-2. Our data reveal a previously unknown cell type–specific regulation of intracellular chloride homeostasis in the perisomatic region of hippocampal pyramidal neurons.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Freund, T.F. & Katona, I. Neuron 56, 33–42 (2007).
Klausberger, T. & Somogyi, P. Science 321, 53–57 (2008).
Földy, C., Lee, S.Y., Szabadics, J., Neu, A. & Soltesz, I. Nat. Neurosci. 10, 1128–1130 (2007).
Klausberger, T., Roberts, J.D. & Somogyi, P. J. Neurosci. 22, 2513–2521 (2002).
Blaesse, P., Airaksinen, M.S., Rivera, C. & Kaila, K. Neuron 61, 820–838 (2009).
Szabadics, J. et al. Science 311, 233–235 (2006).
Duebel, J. et al. Neuron 49, 81–94 (2006).
Inglefield, J.R. & Schwartz-Bloom, R.D. J. Neurosci. Methods 75, 127–135 (1997).
Madison, D.V., Malenka, R.C. & Nicoll, R.A. Nature 321, 695–697 (1986).
Staley, K.J. J. Neurophysiol. 72, 273–284 (1994).
Jentsch, T.J. Crit. Rev. Biochem. Mol. Biol. 43, 3–36 (2008).
Smith, R.L., Clayton, G.H., Wilcox, C.L., Escudero, K.W. & Staley, K.J. J. Neurosci. 15, 4057–4067 (1995).
Sík, A., Smith, R.L. & Freund, T.F. Neuroscience 101, 51–65 (2000).
Nehrke, K. et al. J. Biol. Chem. 277, 23604–23611 (2002).
Bartos, M., Vida, I. & Jonas, P. Nat. Rev. Neurosci. 8, 45–56 (2007).
Acknowledgements
We thank R. Zhu for excellent technical assistance, K. Ding and D. Szabadics for camera lucida reconstructions, M. Case for genotyping, J.E. Melvin for the ClC-2 knockout mouse and L. Hilgenberg, M.A. Smith, M. Cahalan, M.V. Jones, Z. Nusser and R.L. McDonald for discussions. This work was supported by US National Institutes of Health grant NS38580 (to I.S.) and the Epilepsy Foundation through the generous support of the Eric W. Lothman Training Fellowship (to S.-H.L.).
Author information
Authors and Affiliations
Contributions
C.F. and S.-H.L. designed the experiments. C.F., S.-H.L. and R.J.M. performed the experiments and analyzed the data. C.F. and I.S. wrote the manuscript.
Corresponding author
Ethics declarations
Competing interests
The authors declare no competing financial interests.
Supplementary information
Supplementary Text and Figures
Supplementary Figure 1 and Supplementary Methods (PDF 775 kb)
Rights and permissions
About this article
Cite this article
Földy, C., Lee, SH., Morgan, R. et al. Regulation of fast-spiking basket cell synapses by the chloride channel ClC-2. Nat Neurosci 13, 1047–1049 (2010). https://doi.org/10.1038/nn.2609
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/nn.2609
- Springer Nature America, Inc.
This article is cited by
-
Gluconate suppresses seizure activity in developing brains by inhibiting CLC-3 chloride channels
Molecular Brain (2019)
-
Quantitative ultrastructural analysis of basket and axo-axonic cell terminals in the mouse hippocampus
Brain Structure and Function (2015)
-
Identification and characterization of the zebrafish ClC-2 chloride channel orthologs
Pflügers Archiv - European Journal of Physiology (2015)
-
Intracellular chloride concentration influences the GABAA receptor subunit composition
Nature Communications (2012)
-
NMDA receptor activity downregulates KCC2 resulting in depolarizing GABAA receptor–mediated currents
Nature Neuroscience (2011)