Skip to main content

Advertisement

SpringerLink
Log in

Formation of human prostate tissue from embryonic stem cells

  • Brief Communication
  • Published:

From Nature Methods

View current issue Submit your manuscript

Abstract

Rodent models and immortalized or genetically modified cell lines are frequently used—but have limited utility—for studying human prostate development and maturation. Using rodent mesenchyme to establish reciprocal mesenchymal-epithelial cell interactions with human embryonic stem cells (hESCs), we generated human prostate tissue expressing prostate-specific antigen (PSA) within 8–12 weeks. This human prostate model shows species-conserved signalling mechanisms that could extend to integumental, gastrointestinal and genital tissues.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Figure 1: Formation of prostate tissue from hESCs by tissue recombination with UGM or SVM.
Figure 2: Maturation of prostate derived from SVM + hESC after 8–12 weeks growth in intact male hosts.

Similar content being viewed by others

References

  1. vom Saal, F.S. et al. Proc. Natl. Acad. Sci. USA 94, 2056–2061 (1997).

    Article  CAS  Google Scholar 

  2. Donjacour, A.A. & Cunha, G.R. Endocrinology 132, 2342–2350 (1993).

    Article  CAS  Google Scholar 

  3. Cunha, G.R. et al. J. Androl. 13, 465–475 (1992).

    CAS  PubMed  Google Scholar 

  4. Cunha, G.R. et al. Endocr. Rev. 8, 338–362 (1987).

    Article  CAS  Google Scholar 

  5. Risbridger, G. et al. Dev. Biol. 229, 432–442 (2001).

    Article  CAS  Google Scholar 

  6. Higgins, S.J., Young, P., Brody, J.R. & Cunha, G.R. Development 106, 219–234 (1989).

    CAS  PubMed  Google Scholar 

  7. Donjacour, A.A. & Cunha, G.R. Development 121, 2199–2207 (1995).

    CAS  PubMed  Google Scholar 

  8. Reubinoff, B.E., Pera, M.F., Fong, C.Y., Trounson, A. & Bongso, A. Nat. Biotechnol. 18, 399–404 (2000).

    Article  CAS  Google Scholar 

  9. Robaire, B., Ewing, L.L., Irby, D.C. & Desjardins, C. Biol. Reprod. 21, 455–463 (1979).

    Article  CAS  Google Scholar 

  10. Meachem, S.J., McLachlan, R.I., de Kretser, D.M., Robertson, D.M. & Wreford, N.G. Biol. Reprod. 54, 36–44 (1996).

    Article  CAS  Google Scholar 

  11. Teramoto, K. et al. Transplant. Proc. 37, 285–286 (2005).

    Article  CAS  Google Scholar 

  12. Cunha, G.R. et al. J. Cell Biol. 96, 1662–1670 (1983).

    Article  CAS  Google Scholar 

Download references

Acknowledgements

We thank S. Hayward for insightful discussions, and M. Richards and H. Wang for skilled technical assistance. This project was funded by the US Army Department of Defence, Prostate Cancer Research Program (PC020733) to G.P.R. and Perpetual Trustee Company Limited.

Author information

Author notes

  1. Renea A Taylor and Prue A Cowin: These authors contributed equally to this work.

Authors and Affiliations

  1. Centre for Urological Research, Monash Institute of Medical Research, Monash University, 27-31 Wright Street, Clayton, 3168, Victoria, Australia

    Renea A Taylor, Prue A Cowin & Gail P Risbridger

  2. Monash Immunology and Stem Cell Laboratories, STRIP 1, West Ring Road, Monash University, Clayton, 3168, Victoria, Australia

    Renea A Taylor & Alan O Trounson

  3. Department of Anatomy, University of California, San Francisco, 94143, California, USA

    Gerald R Cunha

  4. Australian Stem Cell Centre, P.O. Box 8002, Monash University LPO, Clayton, 3168, Victoria, Australia

    Martin Pera

  5. TissuPath Laboratories, 165 Burwood Road, Hawthorn, 3122, Victoria, Australia

    John Pedersen

Authors
  1. Renea A Taylor
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Prue A Cowin
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Gerald R Cunha
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Martin Pera
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Alan O Trounson
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. John Pedersen
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Gail P Risbridger
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Gail P Risbridger.

Ethics declarations

Competing interests

The authors declare no competing financial interests.

Supplementary information

Supplementary Fig. 1

Glandular regression of prostate tissues derived from SVM + hESC following androgen withdrawal by castration. (PDF 170 kb)

Supplementary Table 1

The proportion of glandular tissue expressing PSA in tissue recombinants following 8-12 weeks growth in vivo. (PDF 87 kb)

Supplementary Methods (PDF 122 kb)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Taylor, R., Cowin, P., Cunha, G. et al. Formation of human prostate tissue from embryonic stem cells. Nat Methods 3, 179–181 (2006). https://doi.org/10.1038/nmeth855

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/nmeth855

  • Springer Nature America, Inc.

This article is cited by

Search

Navigation