Skip to main content
SpringerLink
Log in

On display on a bug: a systematic approach to characterize antibodies

  • News & Views
  • Published:

From Nature Methods

View current issue Submit your manuscript

An Erratum to this article was published on 01 January 2009

This article has been updated

Efficient methods to characterize the binding properties of affinity reagents are required. A combination of bacterial surface display, flow cytometry and pyrosequencing is now used for high-speed mapping of the epitopes recognized by antibodies.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Figure 1: The epitope mapping approach.

Kim Caesar

Change history

  • 26 November 2008

    NOTE: In the version of this article initially published, Figure 1 was incorrectly described as having been adapted from Rockberry et al. The correct citation is Rockberg et al. The error has been corrected in the HTML and PDF versions of the article.

References

  1. Rockberg, J. et al. Epitope mapping of antibodies using bacterial surface display. Nat. Methods 5, 1039–1045 (2008).

    Article  CAS  Google Scholar 

  2. Xu, L. et al. Proc. Natl. Acad. Sci. USA 105, 10756–10761 (2008).

    Article  CAS  Google Scholar 

  3. Binz, H.K., Amstutz, P. & Pluckthun, A. Nat. Biotechnol. 23, 1257–1268 (2005).

    Article  CAS  Google Scholar 

  4. Taussig, M.J. et al. Nat. Methods 4, 13–17 (2007).

    Article  CAS  Google Scholar 

  5. Christmann, A., Wentzel, A., Meyer, C., Meyers, G. & Kolmar, H. J. Immunol. Methods 257, 163–173 (2001).

    Article  CAS  Google Scholar 

  6. Chao, G., Cochran, J.R. & Wittrup, K.D. J. Mol. Biol. 342, 539–550 (2004).

    Article  CAS  Google Scholar 

  7. Uhlen, M. et al. Mol. Cell. Proteomics 4, 1920–1932 (2005).

    Article  CAS  Google Scholar 

  8. Zhu, H. et al. Science 293, 2101–2105 (2001).

    Article  CAS  Google Scholar 

  9. Frank, R. & Overwin, H. Methods Mol. Biol. 66, 149–169 (1996).

    CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Thomas Knorpp and Markus F. Templin are at the Natural and Medical Sciences Institute at the University of Tübingen, Markwiesenstraße 55, 72770 Reutlingen, Germany. templin@nmi.de,

    Thomas Knorpp & Markus F Templin

Authors
  1. Thomas Knorpp
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Markus F Templin
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Knorpp, T., Templin, M. On display on a bug: a systematic approach to characterize antibodies. Nat Methods 5, 1003–1004 (2008). https://doi.org/10.1038/nmeth1208-1003

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1038/nmeth1208-1003

  • Springer Nature America, Inc.

This article is cited by

Search

Navigation