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Proteome expression moves in vitro: resources and tools for harnessing the human proteome

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Comprehensive sets of clones and improved high-throughput methods for production of functional proteins now allow proteome-scale in vitro experiments on nearly 15,000 human genes.

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Figure 1: Schematic of a high-throughput process for proteome-scale in vitro synthesis and characterization of tagged human proteins derived from Gateway entry clones.

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Authors and Affiliations

  1. James L. Hartley is at SAIC-Frederick, National Cancer Institute, Frederick, Maryland 21702, USA. hartley@ncifcrf.gov,

    James L Hartley

  2. Kourosh Salehi-Ashtiani & David E. Hill are at the Center for Cancer Systems Biology and Department of Cancer Biology, Dana-Farber Cancer Institute, Boston, Massachusetts 02115, USA. kourosh_salehi-ashtiani@dfci.harvard.edu david_hill@dfci.harvard.edu,

    Kourosh Salehi-Ashtiani & David E Hill

Authors
  1. James L Hartley
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  2. Kourosh Salehi-Ashtiani
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  3. David E Hill
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Hartley, J., Salehi-Ashtiani, K. & Hill, D. Proteome expression moves in vitro: resources and tools for harnessing the human proteome. Nat Methods 5, 1001–1002 (2008). https://doi.org/10.1038/nmeth1208-1001

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