A novel atomic force microscopy (AFM) setup allows researchers to image and manipulate unsupported membrane proteins separating two aqueous compartments. This promises to permit new detailed measurements of protein conformational changes and interactions under native-like conditions.
References
Engel, A. & Müller, D.J. Nat. Struct. Biol. 7, 715–718 (2000).
Oesterhelt, F., et al. Science 288, 143–146 (2000).
Scheuring, S. & Sturgis, J.N. Science 309, 484–487 (2005).
Müller, D.J. et al. Curr. Opin. Struct. Biol. 16, 489–495 (2006).
Gonçalves, R.P. et al. Nat. Methods 3, 1007–1012 (2006).
Fotiadis, D. et al. Nature 421, 127–128 (2003).
Viani, M.B. et al. Nat. Struct. Biol. 7, 644–647 (2000).
Ando, T. et al. Proc. Natl. Acad. Sci. USA 98, 12468–12472 (2001).
Humphris, A.D.L., Hobbs, J.K. & Miles, M.J. Appl. Phys. Lett. 83, 6–8 (2003).
Hinterdorfer, P. & Dufrêne, Y.F. Nat. Methods 3, 347–355 (2006).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Dufrêne, Y. Atomic force microscopy of membrane proteins separating two aqueous compartments. Nat Methods 3, 973–975 (2006). https://doi.org/10.1038/nmeth1206-973
Issue Date:
DOI: https://doi.org/10.1038/nmeth1206-973
- Springer Nature America, Inc.