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Use of quantum dots for live cell imaging

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Figure 1: The mixed surface approach for conjugating biomolecules to DHLA-capped QDs using avidin as the linker molecule.
Figure 2: Distribution of GM1 gangliosides found in the plasma membrane.

References

  1. Emptage, N.J. Fluorescent imaging in living systems. Curr. Opin. Pharmacol. 1, 521|[ndash]|525 (2001).

    Article  CAS  Google Scholar 

  2. Stephens, D.J. & Allan, V.J. Light microscopy techniques for live cell imaging. Science 300, 82|[ndash]|86 (2003).

    Article  CAS  Google Scholar 

  3. Haugland, R.P. Handbook of Fluorescent Probes and Research Products (Molecular Probes, Eugene, Oregon, USA, 2002).

    Google Scholar 

  4. Jaiswal, J.K., Mattoussi, H., Mauro, J.M. & Simon, S.M. Long-term multiple color imaging of live cells using quantum dot bioconjugates. Nat. Biotechnol. 21, 47|[ndash]|51 (2003).

    Article  CAS  Google Scholar 

  5. Wu, X. et al. Immunofluorescent labeling of cancer marker Her2 and other cellular targets with semiconductor quantum dots. Nat. Biotechnol. 21, 41|[ndash]|46 (2003).

    Article  CAS  Google Scholar 

  6. Mattoussi, H. et al. Self-assembly of CdSe-ZnS quantum dot bioconjugates using an engineered recombinant protein. J. Am. Chem. Soc. 122, 12142|[ndash]|12150 (2000).

    Article  CAS  Google Scholar 

  7. Mattoussi, H. et al. Bioconjugation of highly luminescent colloidal CdSe-ZnS quantum dots with an engineered two-domain recombinant protein. Phys. Stat. Sol. 224, 277|[ndash]|283 (2001).

    Article  CAS  Google Scholar 

  8. Goldman, E.R. et al. Conjugation of luminescent quantum dots with antibodies using an engineered adaptor protein to provide new reagents for fluoroimmunoassays. Anal. Chem. 74, 841|[ndash]|847 (2002).

    Article  CAS  Google Scholar 

  9. Goldman, E.R. et al. Avidin: a natural bridge for quantum dot-antibody conjugates. J. Am. Chem. Soc. 124, 6378|[ndash]|6382 (2002).

    Article  CAS  Google Scholar 

  10. Medintz, I.L. et al. Self-assembled nanoscale biosensors based on quantum dot FRET donors. Nat. Mater. 2, 630|[ndash]|638 (2003).

    Article  CAS  Google Scholar 

  11. Voura, E.B., Jaiswal, J.K., Mattoussi, H. & Simon, S.M. Tracking metastatic tumor cell extravasation with quantum dot nanocrystals and fluorescence emission|[ndash]|scanning microscopy. Nat. Med. 10, 993|[ndash]|998 (2004).

    Article  CAS  Google Scholar 

  12. Larson, D.R. et al. Water-soluble quantum dots for multiphoton fluorescence imaging in vivo . Science 300, 1434|[ndash]|1436 (2003).

    Article  CAS  Google Scholar 

  13. Jaiswal, J.K. & Simon, S.M. Potentials and pitfalls of fluorescent quantum dots for biological applications. Trends Cell Biol. 14, 497|[ndash]|504 (2004).

    Article  CAS  Google Scholar 

  14. Morris, M.C., Depollier, J., Mery, J., Heitz, F. & Divita, G. A peptide carrier for the delivery of biologically active proteins into mammalian cells. Nat. Biotechnol. 19, 1173|[ndash]|1176 (2001).

    Article  CAS  Google Scholar 

  15. Mattheakis, L.C. et al. Optical coding of mammalian cells using semiconductor quantum dots. Anal. Biochem. 327, 200|[ndash]|208 (2004).

    Article  CAS  Google Scholar 

  16. Richard, J.P. et al. Cell-penetrating peptides. A reevaluation of the mechanism of cellular uptake. J. Biol. Chem. 278, 585|[ndash]|590 (2003)

    Article  CAS  Google Scholar 

  17. Dubertret, B. et al. In vivo imaging of quantum dots encapsulated in phospholipid micelles. Science 298, 1759|[ndash]|1762 (2002).

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. The Rockefeller University, Laboratory of Cellular Biophysics, 1230 York Avenue, New York, 10021, New York, USA

    Jyoti K Jaiswal & Sanford M Simon

  2. Center for Bio/Molecular Science and Engineering, United States Naval Research Laboratory, Washington, 20375, DC, USA

    Ellen R Goldman

  3. Division of Optical Sciences, United States Naval Research Laboratory, Washington, 20375, DC, USA

    Hedi Mattoussi

Authors
  1. Jyoti K Jaiswal
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  2. Ellen R Goldman
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  3. Hedi Mattoussi
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  4. Sanford M Simon
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Jaiswal, J., Goldman, E., Mattoussi, H. et al. Use of quantum dots for live cell imaging. Nat Methods 1, 73–78 (2004). https://doi.org/10.1038/nmeth1004-73

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