Abstract
A decade after the introduction of genetically encoded Ca2+ indicator proteins (GECIs), a new generation of improved GECIs demonstrates their usefulness for the functional analysis of the mammalian brain in vivo.
References
Denk, W., Strickler, J.H. & Webb, W.W. Science 248, 73–76 (1990).
Wallace, D.J. et al. Nat. Methods 5, 897–904 (2008).
Mank, M. et al. Nat. Methods 5, 905–911 (2008).
Tsien, R.Y. Annu. Rev. Neurosci. 12, 227–253 (1989).
Svoboda, K., Helmchen, F., Denk, W. & Tank, D.W. Nat. Neurosci. 2, 65–73 (1999).
Stosiek, C., Garaschuk, O., Holthoff, K. & Konnerth, A. Proc. Natl. Acad. Sci. USA 100, 7319–7324 (2003).
Nagayama, S. et al. Neuron 53, 789–803 (2007).
Knöpfel, T., Diez-Garcia, J. & Akemann, W. Trends Neurosci. 29, 160–166 (2006).
Heim, N. et al. Nat. Methods 4, 127–129 (2007).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Rochefort, N., Konnerth, A. Genetically encoded Ca2+ sensors come of age. Nat Methods 5, 761–762 (2008). https://doi.org/10.1038/nmeth0908-761
Issue Date:
DOI: https://doi.org/10.1038/nmeth0908-761
- Springer Nature America, Inc.
This article is cited by
-
The biliary tree—a reservoir of multipotent stem cells
Nature Reviews Gastroenterology & Hepatology (2012)
-
In vivo two-photon imaging of sensory-evoked dendritic calcium signals in cortical neurons
Nature Protocols (2011)