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Sex to the rescue

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Abstract

Applying a classical solution to a cutting-edge problem, two groups used bacterial conjugation to construct Escherichia coli double mutants on a genome-wide scale. This will allow comprehensive genetic interaction screens in bacteria for the first time.

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Figure 1: Creating double mutants in E. coli by conjugation.

Katie Ris-Vicari

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Authors and Affiliations

  1. Thomas J. Silhavy and Zemer Gitai are in the Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA. tsilhavy@princeton.edu,

    Thomas J Silhavy & Zemer Gitai

Authors
  1. Thomas J Silhavy
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  2. Zemer Gitai
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Silhavy, T., Gitai, Z. Sex to the rescue. Nat Methods 5, 759–760 (2008). https://doi.org/10.1038/nmeth0908-759

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