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Valid data from large-scale proteomics studies

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How sure can we be to have identified the right proteins in a large scale proteomics study with our mass spectrometric instrumentation? Can we expect valid data from the employed search algorithm(s)? Can we believe what our computer is telling us? Right questions—what are the answers?

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Figure 1: Illustration of the difficulty in identifying the same proteins using different MS platforms.

Katie Ris

References

  1. Elias, J.E., Haas, W., Faherty, B.K. & Gygi, S.P. Nat. Methods 2, 667–675 (2005).

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Authors and Affiliations

  1. Protagen AG, Dortmund, Germany

    Daniel Chamrad & Helmut E Meyer

  2. Helmut E. Meyer is at Ruhr University of Bochum, Bochum, Germany

    Daniel Chamrad & Helmut E Meyer

Authors
  1. Daniel Chamrad
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  2. Helmut E Meyer
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Chamrad, D., Meyer, H. Valid data from large-scale proteomics studies. Nat Methods 2, 647–648 (2005). https://doi.org/10.1038/nmeth0905-647

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