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EGFP as your targeted 'hitman'

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The nonphototoxic nature of GFP makes it an excellent imaging probe but a poor tool for techniques that rely on generation of toxic radicals such as chromophore-assisted light inactivation (CALI). Multiphoton excitation helps overcome these limitations.

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Figure 1: Three ways by which a 'killing' chromophore is introduced into cells and attached to a target protein (yellow oval).

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  1. Howard Hughes Medical Institute associate at the Department of Pharmacology, University of California, San Diego, 9500 Gilman Drive, La Jolla, 92093, California, USA

    Oded Tour

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  1. Oded Tour
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Tour, O. EGFP as your targeted 'hitman'. Nat Methods 2, 491–492 (2005). https://doi.org/10.1038/nmeth0705-491

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