Skip to main content
SpringerLink
Log in

Without a trace? PiggyBac-ing toward pluripotency

  • News & Views
  • Published:

From Nature Methods

View current issue Submit your manuscript

A transposon-based approach has been added to the growing arsenal of technologies to produce transgene-free and potentially safer induced pluripotent stem cells.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Figure 1: Strategies to generate transgene-free iPSCs.

References

  1. Yusa, K., Rad, R., Takeda, J. & Bradley, A. Nat. Methods 6, 363–369 (2009).

    Article  CAS  PubMed Central  Google Scholar 

  2. Takahashi, K. & Yamanaka, S. Cell 126, 663–676 (2006).

    Article  CAS  PubMed Central  Google Scholar 

  3. Maherali, N. & Hochedlinger, K. Cell Stem Cell 3, 595–605 (2008).

    Article  CAS  PubMed Central  Google Scholar 

  4. Soldner, F. et al. Cell 136, 964–977 (2009).

    Article  CAS  PubMed Central  Google Scholar 

  5. Stadtfeld, M., Nagaya, M., Utikal, J., Weir, G. & Hochedlinger, K. Science 322, 945–949 (2008).

    Article  CAS  PubMed Central  Google Scholar 

  6. Okita, K., Nakagawa, M., Hyenjong, H., Ichisaka, T. & Yamanaka, S. Science 322, 949–953 (2008).

    Article  CAS  PubMed Central  Google Scholar 

  7. Yu, J. et al. Science advance online publication, doi:10.1126/science.1172482 (26 March 2009).

    Google Scholar 

  8. Kaji, K. et al. Nature advance online publication, doi:10.1038/nature07864 (1 March 2009).

  9. Woltjen, K. et al. piggyBac transposition reprograms fibroblasts to induced pluripotent stem cells. Nature advance online publication, doi:10.1038/nature07863 (1 March 2009).

    Google Scholar 

  10. Wang, W. et al. Proc. Natl. Acad. Sci. USA 105, 9290–9295 (2008).

    Article  CAS  Google Scholar 

  11. Geurts, A.M. et al. PLoS Genet. 2, e156 (2006).

    Article  PubMed Central  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Matthias Stadtfeld and Konrad Hochedlinger are in the Department of Medicine, Department of Stem Cell and Regenerative Biology, Cancer Center and Center for Regenerative Medicine, Massachusetts General Hospital, Boston, Harvard University, Cambridge, Massachusetts, USA. khochedlinger@helix.mgh.harvard.edu or mstadtfeld@mgh.harvard.edu,

    Matthias Stadtfeld & Konrad Hochedlinger

Authors
  1. Matthias Stadtfeld
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Konrad Hochedlinger
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Stadtfeld, M., Hochedlinger, K. Without a trace? PiggyBac-ing toward pluripotency. Nat Methods 6, 329–330 (2009). https://doi.org/10.1038/nmeth0509-329

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1038/nmeth0509-329

  • Springer Nature America, Inc.

Search

Navigation