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Two-photon dual-color imaging using fluorescent proteins

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Figure 1: Dual-color imaging of two pairs of fluorescent proteins under a two-photon laser scanning microscope.

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Acknowledgements

This work was partly supported by grants from the Japanese Ministry of Education, Culture, Sports, Science and Technology Grant-in-Aid for Scientific Research on priority areas, the Japan New Energy and Industrial Technology Development Organization, and the Human Frontier Science Program.

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Authors and Affiliations

  1. Laboratory for Cell Function and Dynamics, Brain Science Institute, RIKEN, 2-1 Hirosawa, Wako-city, 351-0198, Saitama, Japan

    Hiroyuki Kawano, Takako Kogure, Hideaki Mizuno & Atsushi Miyawaki

  2. Life Function and Dynamics, Exploratory Research for Advanced Technology, Japan Science and Technology Agency, 2-1 Hirosawa, 351-0198, Wako-city, Saitama, Japan

    Hiroyuki Kawano, Yukiko Abe & Atsushi Miyawaki

Authors
  1. Hiroyuki Kawano
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  2. Takako Kogure
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  3. Yukiko Abe
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  4. Hideaki Mizuno
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  5. Atsushi Miyawaki
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Correspondence to Atsushi Miyawaki.

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Competing interests

A.M. is a member of the scientific advisory board of Amalgaam, which handles the distribution of mKeima.

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Supplementary Figures 1–2, Supplementary Methods (PDF 102 kb)

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Kawano, H., Kogure, T., Abe, Y. et al. Two-photon dual-color imaging using fluorescent proteins. Nat Methods 5, 373–374 (2008). https://doi.org/10.1038/nmeth0508-373

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